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Shodex Asahipak GS and GF Series_43.jpg

Asahipak GF
columns

separation by size for a variety of molecular weights

The polymer-based SEC (GFC) columns for aqueous/organic solvents

Product information

Asahipak GF series [multi-solvent columns]

  • Polymer-based SEC columns with high solvent durability

  • Works well with both aqueous and organic solvents

Usable solvents for Asahipak GF-210 HQ, GF-310 HQ, GF-510 HQ, GF-7M HQ:

Water (0 - 0.5 M salt conc.)

Different Buffers

Methanol

Ethanol

Acetonitrile

THF

DMF

Acetone

Chloroform

Ethyl acetate

DMSO (0 to 50 %)

Product overview

Product code
Product name
Separation
Functional group
Gel material
Plates per column
Particle size
Pore size
Size (ID x length)
Housing material
F7600000
Asahipak GF-210 HQ
SEC
-
Polyvinyl alcohol
≥ 19,000
5 µm
180 Å
7.5 x 300 mm
steel
F7600001
Asahipak GF-310 HQ
SEC
-
Polyvinyl alcohol
≥ 19,000
5 µm
400 Å
7.5 x 300 mm
steel
F7600002
Asahipak GF-510 HQ
SEC
-
Polyvinyl alcohol
≥ 19,000
5 µm
2,000 Å
7.5 x 300 mm
steel
F7600004
Asahipak GF-7M HQ
SEC
-
Polyvinyl alcohol
≥ 13,000
9 µm
10,000 Å
7.5 x 300 mm
steel
F6710018
Asahipak GF-1G 7B
SEC
-
Polyvinyl alcohol
(guard)
9 µm
-
7.5 x 50 mm
steel
F6810038
Asahipak GS-310 20G
SEC
-
Polyvinyl alcohol
≥ 14,000
13 µm
400 Å
20 x 500 mm
steel
F6710020
Asahipak GS-10G 7B
SEC
-
Polyvinyl alcohol
(guard)
20 µm
-
7.5 x 50 mm
steel

Product details

Product name
Maximum pressure
Usual flow rate
Maximum flow rate
Temperature range
pH range
Salt concentration
Shipping solvent
USP
MW range
Exclusion limit
Asahipak GF-210 HQ
9 MPa (90 bar)
0.4 to 0.6 mL/min
1.0 mL/min
4 to 60°C
2 to 9
≤ 0.5 M
H2O
-
300 - 4,000
9,000
Asahipak GF-310 HQ
7 MPa (70 bar)
0.4 to 0.6 mL/min
1.0 mL/min
4 to 60°C
2 to 9
≤ 0.5 M
H2O/CH3OH = 70/30
-
300 - 30,000
40,000
Asahipak GF-510 HQ
6.5 MPa (65 bar)
0.4 to 0.6 mL/min
1.0 mL/min
4 to 60°C
2 to 9
≤ 0.5 M
H2O/CH3OH = 70/30
-
5,000 - 200,000
300,000
Asahipak GF-7M HQ
4.5 MPa (45 bar)
0.4 to 0.6 mL/min
1.0 mL/min
4 to 60°C
2 to 9
≤ 0.5 M
H2O/CH3OH = 70/30
-
300 - (10,000,000)
(10,000,000)
Asahipak GF-1G 7B
-
-
-
4 to 60°C
2 to 9
≤ 0.5 M
H2O/CH3OH = 70/30
-
-
-
Asahipak GS-310 20G
2 MPa (20 bar)
5.0 to 8.0 mL/min
12 mL/min
4 to 60°C
2 to 9
≤ 0.5 M
H2O/CH3OH = 70/30
-
300 - 30,000
40,000
Asahipak GS-10G 7B
-
-
-
4 to 60°C
2 to 9
≤ 0.5 M
H2O/CH3OH = 70/30
-
-
-

Product info

Applications

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Calibration curves

Calibration Curves for GF-HQ Series (Acetone Eluent: PMMA)

Calibration Curves for GF-HQ Series (Aqueous Eluent: PEG, PEO)

Calibration Curves for GF-HQ Series (Aqueous Eluent: Proteins)

Calibration Curves for GF-HQ Series (Aqueous Eluent: Pullulan)

Calibration Curves for GF-HQ Series (Chloroform Eluent: PS)

Calibration Curves for GF-HQ Series (DMF Eluent: PEG, PEO)

Calibration Curves for GF-HQ Series (DMF Eluent: PMMA)

Calibration Curves for GF-HQ Series (DMF Eluent: PS)

Calibration Curves for GF-HQ Series (Methanol Eluent: PEG, PEO)

Calibration Curves for GF-HQ Series (THF Eluent: PS)

Calibration Curve for GF-510 HQ + GF-310 HQ (DMF Eluent: PS)

Calibration Curve for GF-510 HQ + GF-310 HQ (THF Eluent: PS)

Calibration Curves of Hydrophilic Polymers (1) (Sodium Poly(Styrene Sulfonate))

Calibration Curves of Hydrophilic Polymers (2) (Sodium Polyacrylate)

Calibration Curves of Hydrophilic Polymers (3) (Poly(Sodium Methacrylate))

 

Asahipak GF-210 HQ

Analysis of Various Tween Type Surfactants (GF-210 HQ)

Comparison of maltooligosaccharides separation

Comparison of Triglycerides Separation

Method Optimization for The Simultaneous Analysis of Anionic and Non-ionic Surfactants (GF-210 HQ)

Triton X-100 (GF-210 HQ)

 

Asahipak GF-310 HQ

Analysis Method for Surfactants

Adrenal Cortical Hormones (2) (GF-310 HQ)

Analysis of High Molecular Weight Hindered Amine Light Stabilizers (HALS) (GF-310 HQ)

Analysis of Uric Acid and Creatinine using Column Switching Method

Benzalkonium Chloride (1) (GF-310 HQ)

Benzalkonium Chloride (2) (GF-310 HQ)

Brij® 30 (Polyoxyethylene(4) Lauryl Ether) (1) (GF-310 HQ)

Brij® 56 (Polyoxyethylene(10) Cetyl Ether) (GF-310 HQ)

Comparison of maltooligosaccharides separation

Comparison of Triglycerides Separation

Comparison with ODS Column (1)

Comparison with ODS Column (2)

Comparison with ODS Column (3)

Conjugated Lipid (1) (Lecithin from Soybean) (GF-310 HQ)

Conjugated Lipid (2) (L-α-Phosphatidylethanolamine, Dioleoyl) (GF-310 HQ)

Degree of POE Polymerization (GF-310 HQ)

Dialkyltin Compounds (GS-310 7E)

Effects of Analysis of PEG Mixtures with GF-310 HQ by Composition of Eluent

Effects of Organic Solvent Concentration (1) (GF-310 HQ)

Effects of Organic Solvent Concentration (2)

Effects of Organic Solvents (GF-310 HQ)

Effects of Temperature (GF-310 HQ)

Epoxy Resin (3) (Mixed Solvent Eluent)

Glycerides (1) (Chloroform Eluent) (GF-310 HQ)

Glycerides (2) (THF Eluent) (GF-310 HQ)

Glycerides (3) (DMF Eluent) (GF-310 HQ)

Hydrophile-Lipophile Balance (HLB)

Hydrophobicity of Columns

Influence of Column Temperature on Retention Time of PEG (GF-310 HQ and GS-320 HQ)

Influence of Salt Concentration on Retention Time of PEG with GF-310 HQ Column

Melamine-Formaldehyde Resin Prepolymers (Mixed Solvent Eluent)

Microchemical Analysis of Cationic Surfactants (GF-310 HQ)

Nonylphenol Ethoxylates (1) (GF-310 HQ)

p-Cresol Formaldehyde Resol Resin (Mixed Solvent Eluent)

Phenol Formaldehyde Novolac Resin (Mixed Solvent Eluent)

Poly(o-Cresyl Glycidyl Ether)-co-Formaldehyde (GF-310 HQ)

Polyoxethylene Sorbitan Monoleate (GF-310 HQ)

Polystyrene Standards (21) (GF-HQ : THF, Chloroform and DMF)

Pullulan Standards (2) (GF-310 HQ)

Recovery of Proteins (GF-310 HQ and GS-320 HQ)

Sodium Alkyl Sulfates (GF-310 HQ)

Sodium Dodecylbenzenesulfonate (1) (GF-310 HQ)

Sodium Dodecylbenzenesulfonate (2) (GF-310 HQ)

Triglycerides (GF-310 HQ)

Trimethylalkylammonium Chlorides (GF-310 HQ)

Triton X-100 (GF-310 HQ)

 

Asahipak GF-510 HQ

Ammonio Methacrylate Copolymer (GF-510 HQ)

Comparison of Chromatograms of Protein mixtures

Crude γ Phage DNA Fragments

Hydrophobicity of Columns

Polystyrene Standards (21) (GF-HQ : THF, Chloroform and DMF)

Preparation of CS-Glucan in Coix Seed

Sodium Poly(Styrene Sulfonate) (1) (GF-510 HQ)

Standard Proteins (23) (GF-510 HQ)

 

Asahipak GF-7M HQ

Biodegradable Polymer (1) (Poly(Lactic-co-Glycolic Acid)) (GF-7M HQ)

Optimization of Mobile Phase for the Analysis of Sodium Polyacrylate (GF-7M HQ)

Poly(Sodium Methacrylate) (GF-7M HQ)

Pullulan Standards (9) (GF-7M HQ)

Selection of Column (1) (Sodium Poly(Styrene Sulfonate))

Selection of Column (2) (Sodium Polyacrylate)

Selection of Column (3) (Poly(Sodium Methacrylate))

Sodium Poly(Styrene Sulfonate) (2) (GF-7M HQ)

Related products

Shodex STANDARD P-82 (Pullulan) is a calibration standard kit for aqueous size exclusion chromatography.

Pullulan is an unbranched polysaccharide: units of maltotriose are connected with α-1,6 bond.

  • Easy to prepare

  • For aqueous SEC (GFC)

  • Unbranched pullulan standard

  • High solubility in water eliminates the possibility of recrystallization

STANDARD P-82 kit consists of 8 vials with different molecular weight standards of 200 mg each:

MW 800.000, 400.000, 200.000, 100.000, 50.000, 20.000, 10.000, 5.000​

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