Asahipak ODP-50 columns

with C18 groups

The robust polymer-based RP column for many general applications

Product information

Asahipak ODP-50

The Shodex Asahipak ODP-50 series are columns for reversed phase chromatography. These columns are packed with spherical porous polymer having octadecyl (C18) groups, and perform similar to general silica-based C18 ODS columns. These columns are compatible with not only water or polar organic solvents, but also the wide pH range between 2 to 13. There are ideal for LC-MS/MS analysis.

Relatively large pore size of 250 A is suitable for the analysis of amino acids, peptides, and proteins
Usable in a wide pH range from pH 2 to 13
Usable in 100 % water and buffer solution
Best used for the analysis of basic substances
Fulfills USP L67 requirements
Also available as Asahipak C4P-50 with C4 groups
Also suitable for evaporative light scattering detector (ELSD), corona charged aerosol detector (CAD), and mass spectrometry (LC-MS/MS)

Over 60% of all HPLC applications are Reversed Phase (RP) separations with C18 functional groups. Spherical silica particles proved their use as base material in HPLC columns for many years. To minimize the negative effects of residual silanol groups on the surface, researchers tried end-capping or polymeric coating of the particle and developed hybrid materials.

Here we introduce an alternative for these ODS (octadecyl-bonded silica) RP columns:
A polymer particle (for example polyvinyl alcohol) with 5 μm particle size is modified with C18 alkyl chains as functional groups and used as stationary phase. This HPLC column has greater long-term pH stability from 2 to 13. It contains no silanol groups and provides higher resolution for basic substances, for example drugs with tertiary amines (no peak tailing due to unwanted interactions).

Also the polarity of the polymer gel is slightly more hydrophilic which gives more retention of polar compounds at the beginning of the chromatogram. The polymer-based RP material can be used in 100% water or buffer and a variety of organic solvents (isocratic and gradient separation). It has a pore size of 250 Å which makes it suitable for the analysis of proteins and peptides.

The very low bleeding is advantageous for the hyphenation to mass spectrometry (MS) or particle-sensitive light scattering detectors. A C18 column with a stationary phase made of polymer offers more than double the life time compared to silica, because it is chemically more resistant.

Shodex is specialized in polymer-based packing material for Reversed Phase chromatography. The modern column Asahipak ODP-50 (octadecyl-bonded polymer) combines all the advantages of a polymeric RP-C18 column. It is used for small organic molecules, pharmaceuticals, pesticides, hormones, proteins, vitamins and basic drugs.

Product overview

Product code	Product name	Separation	Functional group	Gel material	Plates per column	Particle size	Pore size	Size (ID x length)	Housing material
F7620009	Asahipak ODP-50 2D	RP	C18	Polyvinyl alcohol	≥ 5,000	5 µm	250 Å	2.0 x 150 mm	steel
F6713001	Asahipak ODP-50G 2A	RP	C18	Polyvinyl alcohol	(guard)	5 µm	-	2.0 x 10 mm	steel
F6710023	Asahipak ODP-50 4B	RP	C18	Polyvinyl alcohol	≥ 2,500	5 µm	250 Å	4.6 x 50 mm	steel
F7620004	Asahipak ODP-50 4D	RP	C18	Polyvinyl alcohol	≥ 9,000	5 µm	250 Å	4.6 x 150 mm	steel
F7620003	Asahipak ODP-50 4E	RP	C18	Polyvinyl alcohol	≥ 14,000	5 µm	250 Å	4.6 x 250 mm	steel
F6710022	Asahipak ODP-50G 4A	RP	C18	Polyvinyl alcohol	(guard)	5 µm	-	4.6 x 10 mm	steel
F7620002	Asahipak ODP-50 6D	RP	C18	Polyvinyl alcohol	≥ 9,000	5 µm	250 Å	6.0 x 150 mm	steel
F7620001	Asahipak ODP-50 6E	RP	C18	Polyvinyl alcohol	≥ 14,000	5 µm	250 Å	6.0 x 250 mm	steel
F6710001	Asahipak ODP-50G 6A	RP	C18	Polyvinyl alcohol	(guard)	5 µm	-	6.0 x 10 mm	steel
F6820001	Asahipak ODP-50 10E	RP	C18	Polyvinyl alcohol	≥ 10,000	5 µm	250 Å	10.0 x 250 mm	steel
F7620008	Asahipak C4P-50 4D	RP	C4	Polyvinyl alcohol	≥ 6,000	5 µm	250 Å	4.6 x 150 mm	steel
F6710003	Asahipak C4P-50G 4A	RP	C4	Polyvinyl alcohol	(guard)	5 µm	-	4.6 x 10 mm	steel

Product details

Product name	Maximum pressure	Usual flow rate	Maximum flow rate	Temperature range	pH range	Salt concentration	Shipping solvent	USP	Info
Asahipak ODP-50 2D	12 MPa (120 bar)	0.2 mL/min	0.4 mL/min	4 to 60°C	2 to 13	≤ 0.1 M	H2O/ACN = 35/65	L67	Carbon load 17%
Asahipak ODP-50G 2A	-	-	-	4 to 60°C	2 to 13	≤ 0.1 M	H2O/ACN = 35/65	L67	Carbon load 17%
Asahipak ODP-50 4B	5.0 MPa (50 bar)	0.4 to 0.6 mL/min	1.5 mL/min	4 to 60°C	2 to 13	≤ 0.1 M	H2O/ACN = 35/65	L67	Carbon load 17%
Asahipak ODP-50 4D	15 MPa (150 bar)	0.5 to 0.8 mL/min	1.5 mL/min	4 to 60°C	2 to 13	≤ 0.1 M	H2O/ACN = 35/65	L67	Carbon load 17%
Asahipak ODP-50 4E	15 MPa (150 bar)	0.5 to 0.8 mL/min	1.5 mL/min	4 to 60°C	2 to 13	≤ 0.1 M	H2O/ACN = 35/65	L67	Carbon load 17%
Asahipak ODP-50G 4A	-	-	-	4 to 60°C	2 to 13	≤ 0.1 M	H2O/ACN = 35/65	L67	Carbon load 17%
Asahipak ODP-50 6D	15 MPa (150 bar)	0.5 to 1.0 mL/min	2.5 mL/min	4 to 60°C	2 to 13	≤ 0.1 M	H2O/ACN = 35/65	L67	Carbon load 17%
Asahipak ODP-50 6E	15 MPa (150 bar)	0.5 to 1.0 mL/min	2.5 mL/min	4 to 60°C	2 to 13	≤ 0.1 M	H2O/ACN = 35/65	L67	Carbon load 17%
Asahipak ODP-50G 6A	-	-	-	4 to 60°C	2 to 13	≤ 0.1 M	H2O/ACN = 35/65	L67	Carbon load 17%
Asahipak ODP-50 10E	15 MPa (150 bar)	1.5 to 2.0 mL/min	3.0 mL/min	4 to 60°C	2 to 13	≤ 0.1 M	H2O/ACN = 35/65	L67	Carbon load 17%
Asahipak C4P-50 4D	15 MPa (150 bar)	0.5 to 0.8 mL/min	1.0 mL/min	4 to 60°C	2 to 13	≤ 0.1 M	H2O/ACN = 35/65	-	Carbon load 6%
Asahipak C4P-50G 4A	-	-	-	4 to 60°C	2 to 13	≤ 0.1 M	H2O/ACN = 35/65	-	Carbon load 6%

Download documents

Shodex Technical Article 14_Analysis of Functional Ingredients in Dietary Supplement (pdf)

Shodex Application Note Polymer RP-C18 Asahipak ODP-50 (pdf)

Operation Manual Asahipak C4P-50 4D_20221202E

Operation Manual Asahipak ODP-50 2D_20221202E

Operation Manual Asahipak ODP-50 4B, 4D, 4E_20221202E

Operation Manual Asahipak ODP-50 6D, 6E_20221202E

Operation Manual Asahipak ODP-50 10E_prep_20221202E

Bedienungsanleitung Asahipak C4P-50 4D_20221202G

Bedienungsanleitung Asahipak ODP-50 2D_20221202G

Bedienungsanleitung Asahipak ODP-50 4B, 4D, 4E_20221202G

Bedienungsanleitung Asahipak ODP-50 6D, 6E_20221202G

Bedienungsanleitung Asahipak ODP-50 10E_prep_20221202G

Product instruction

Eluents:

It is possible to use pure water, methanol and acetonitrile 100 % each, and any proportion of these solvents. Acetonitrile is recommended in case of low resolution of aromatic substances with methanol containing eluent.

It is possible to use buffer agents like phosphoric salt, acetate salt and Tris-HCI. And neutral salts such as NaCl, KCI, Na2SO4 and acids such as trifluoroacetic acid are also usable.

Cleaning:

Elution characteristics of a column may change considerably after long, repeated usage, due to the accumulation of sample microadsorbents on the packing material surface. In such cases, remove guard column and set analysis column with reverse direction with its flow direction, cleaning the column with 50 mL or more of acetonitrile or methanol may be effective for recovery of column efficiency. Guard column should be cleaned alone with the same way. The flow rate should be applied lower than 0.5 mL/min.

Applications

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General

Comparison of Silica-based Column with Polymer-based Column

Durability of ODP-50 Column with Alkaline Eluent

Effects of Eluent pH (ODP-50)

Effects of pH of Eluent on Elution Volume and Plate Number (ODP-50)

Effects of pH of Eluent on Sensitivity of Barbital (ODP-50 4D)

Preservative

2-Phenoxyethanol (ODP-50 4D)

Preservatives (ODP-50 4D)

Other

Anilines (ODP-50 6D)

Aliphatic Alcohols (3) (ODP-50 6D, C4P-50 6D)