Chiral ORpak CDBS-453 column
with β-Cyclodextrin derivatives
For the separation of Epinephrine and other chiral drugs
Product information
In the fields of medicine, agricultural chemicals and food, it is important to analyze optical isomers to confirm physiological activity or to measure optical purity of optical isomers. Shodex offers one type of separation column of chiral separation.
ORpak CDBS-453
The ORpak CDBS-453 column is packed with silica-based gel bonded with β-cyclodextrin derivatives as ligand.
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Separates optical isomers by using their conformational compatibility differences
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Versatile column for chiral separation
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Fulfills USP L45 requirements
Analytes: Epinephrine, Norepinephrine, Lamivudine, Voriconazole and other chiral drugs
Example:
Sample: 20 μL Epinephrine (racemic mixture) 50 μg/mL (in eluent)
Column: Shodex ORpak CDBS-453 (4.6 x 150 mm)
Eluent: (0.05 % (w/v) CH3COOH + 0.2 M NaCl) aq./CH3CN=95/5
Flow rate: 0.5 mL/min
Detector: UV (280 nm)
Column temp.: 10°C
Product overview
Product code | Product name | Separation | Functional group | Gel material | Particle size | Pore size | Size (ID x length) | Housing material |
|---|---|---|---|---|---|---|---|---|
F7146003 | ORpak CDBS-453 | Chiral | beta-Cyclodextrin derivate | Silica | 3 µm | - | 4.6 x 150 mm | steel |
Product details
Product name | Maximum pressure | Maximum flow rate | Temperature range | pH range | Salt concentration | Shipping solvent | USP |
|---|---|---|---|---|---|---|---|
ORpak CDBS-453 | 20 MPa (200 bar) | 1.2 mL/min | 5 to 60°C | 2 to 7.5 | ≤ 1.0 M | (0.05 % CH3COOH + 0.2 M NaCl aq.)/CH3CN/ = 95/5 | L45 |
Product info
Product instruction
Eluents:
The separation performance of CDBS-453 will be largely affected by the eluent conditions. A buffer, an organic solvent or a mixture of buffer and organic solvent can be used as an eluent. Commonly, a mixture is used as an eluent.
The following are buffers and organic solvents usually used.
Buffer
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Acetic buffer
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Phosphate buffer
The buffer pH range should be between 2 and 7.
When a salt is added, the salt concentration should be lower than 1.0 M.
When acetic acid is added, the acid concentration should be lower than 6 % (w/v).
Organic solvent
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Acetonitrile
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Methanol
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Isopropyl alcohol
Guard column:
A guard column is not available.
Cleaning and Regeneration:
There is no appropriate cleaning method.
Applications
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Analysis of Epinephrine Injection Proposed in USP-NF Pharmacopeial Forum (CDBS-453)
Chiral Separation of Norepinephrine (CDBS-453)
Impurity Analysis of Lamivudine According to USP-NF Method (CDBS-453)
Impurity Analysis of Voriconazole According to USP-NF Method (CDBS-453)