HILICpak VN-50 columns
with diol groups
The robust HILIC column for oligosaccharides and oligonucleotides
Product information
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The modified diol groups on the packing material create the HILIC mode
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Suitable for oligosaccharide and oligonucleotide separation which is not possible by SEC column or conventional HILIC columns
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Polymer-based packing material provides excellent chemical stability and minimum deterioration over an extended time period
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pH stability: 2 to 13 (silanol free)
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Easily regenerated by washing in an alkaline solution
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Also suitable for evaporative light scattering detector (ELSD), corona charged aerosol detector (CAD), and mass spectrometry (LC-MS/MS)
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New size HILICpak VN-50 1D adopts a double structure housing with an outer surface made of SUS and an inner surface [wetted part] made of PEEK. Covers the weak point of physical strength of PEEK housing and can be used under high pressure conditions. It has an inner diameter of 1.0 mm, which makes it possible to suppress the flow rate to 0.1 mL/min or less, so it suitable for high sensitivity analysis by LC/MS.
Product overview
Product code | Product name | Separation | Functional group | Gel material | Plates per column | Particle size | Pore size | Size (ID x length) | Housing material |
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F7630500 | HILICpak VN-50 4D | HILIC | Diol | Polyvinyl alcohol | ≥ 10,000 | 5 µm | 100 Å | 4.6 x 150 mm | PEEK |
F6711400 | HILICpak VN-50G 4A | HILIC | Diol | Polyvinyl alcohol | (guard) | 5 µm | - | 4.6 x 10 mm | PEEK |
F7630600 | HILICpak VN-50 2D | HILIC | Diol | Polyvinyl alcohol | ≥ 3,500 | 5 µm | 100 Å | 2.0 x 150 mm | PEEK |
F6711500 | HILICpak VN-50G 2A | HILIC | Diol | Polyvinyl alcohol | (guard) | 5 µm | - | 2.0 x 10 mm | PEEK |
F7630800 | HILICpak VN-50 1D | HILIC | Diol | Polyvinyl alcohol | ≥ 2,800 | 5 µm | 100 Å | 1.0 x 150 mm | PEEK |
F6830100 | HILICpak VN-50 10E | HILIC | Diol | Polyvinyl alcohol | ≥ 11,000 | 5 µm | 100 Å | 10.0 x 250 mm | steel |
Product details
Product name | Maximum pressure | Usual flow rate | Maximum flow rate | Temperature range | pH range | Shipping solvent | USP |
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HILICpak VN-50 4D | 10 MPa (100 bar) | 0.3 to 1.0 mL/min | 1.5 mL/min | 4 to 60°C | 2 to 13 | H2O/CH3CN = 25/75 | - |
HILICpak VN-50G 4A | - | 0.3 to 1.0 mL/min | 1.5 mL/min | 4 to 60°C | 2 to 13 | H2O/CH3CN = 25/75 | - |
HILICpak VN-50 2D | 10 MPa (100 bar) | 0.1 to 0.3 mL/min | 0.5 mL/min | 4 to 60°C | 2 to 13 | H2O/CH3CN = 25/75 | - |
HILICpak VN-50G 2A | - | 0.05 to 0.3 mL/min | 0.5 mL/min | 4 to 60°C | 2 to 13 | H2O/CH3CN = 25/75 | - |
HILICpak VN-50 1D | 10 MPa (100 bar) | 0.01 to 0.05 mL/min | 0.1 mL/min | 4 to 60°C | 2 to 13 | H2O/CH3CN = 25/75 | - |
HILICpak VN-50 10E | 15 MPa (150 bar) | 2.0 to 3.0 mL/min | 5.0 mL/min | 4 to 60°C | 2 to 13 | H2O/CH3CN = 25/75 | - |
Product info
Download documents
Technical Articles:
Shodex Technical Article 4_LC-MS Analysis of Oligonucleotides (pdf)
Shodex Technical Article 7_Analysis of Various Oligosaccharides Using HILIC Mode (pdf)
Shodex Technical Article 19_LC-MS Analysis of Various Oligonucleic Acids (pdf)
Applications
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HILICpak VN-50 4D
Analysis of Maltodextrin (VN-50 4D)
Analysis of Oligogalacturonic Acids (VN-50 4D)
Chitosan-Oligosaccharides (Chitooligosaccharides) (3) (VN-50 4D)
Hydrolyzed Dextran (6) (VN-50 4D)
Hydrolyzed Dextran (7) (VN-50 4D by Gradient Elution)
N-Acetylchitooligosaccharides (2) (VN-50 4D)
Rapid Analysis of Trivalent Chromium and Hexavalent Chromium (VN-50 4D)
Separation of Substances Produced During Utilization of Biomass (VN-50 4D)
Xylooligosaccharides (2) (VN-50 4D)
HILICpak VN-50 2D
Analysis of 10 - 50mer Oligo-DNAs (VN-50 2D)
Analysis of 2'-OMe and 2'-MOE Modified Phosphorothioated Oligo-RNA by LC/UV/MS (VN-50 2D)
Analysis of Oligo-DNAs (VN-50 2D)
Analysis of Oligonucleotides and Their Impurities (1) Truncated Oligonucleotides (VN-50 2D)
Analysis of Oligonucleotides and Their Impurities (2) Base Alteration (VN-50 2D)
Analysis of Phosphorothioated Oligo-DNA (VN-50 2D)
Analysis of Various Oligonucleotides by LC/UV/MS (VN-50 2D)
LC/UV/MS Analysis of Oligo-DNA (VN-50 2D)
HILICpak VN-50 1D