OHpak LB-800 columns
separation by size for a variety of molecular weights
For MALS: low-bleeding, polymer-based SEC (GFC) column for modified proteins, PEGs, polysaccharides, surfactants
Product information
OHpak LB-800 series [for MALS]
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Polymer-based packed columns for aqueous SEC (GFC) analysis
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Low column bleeding allows its use with light scattering detectors
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The eluent can be replaced with DMF enabling the analysis of polar polymers
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LB-802.5 (exclusion limit: about 10,000) newly added to the series
Product overview
Product code | Product name | Separation | Functional group | Gel material | Plates per column | Particle size | Pore size | Size (ID x length) | Housing material |
|---|---|---|---|---|---|---|---|---|---|
F6429206 | OHpak LB-802.5 | SEC | - | Polyhydroxy-methacrylate | ≥ 16,000 | 6 µm | 200 Å | 8.0 x 300 mm | steel |
F6429201 | OHpak LB-803 | SEC | - | Polyhydroxy-methacrylate | ≥ 16,000 | 6 µm | 800 Å | 8.0 x 300 mm | steel |
F6429204 | OHpak LB-804 | SEC | - | Polyhydroxy-methacrylate | ≥ 16,000 | 10 µm | 2,000 Å | 8.0 x 300 mm | steel |
F6429203 | OHpak LB-805 | SEC | - | Polyhydroxy-methacrylate | ≥ 12,000 | 13 µm | 7,000 Å | 8.0 x 300 mm | steel |
F6429205 | OHpak LB-806 | SEC | - | Polyhydroxy-methacrylate | ≥ 12,000 | 13 µm | 15,000 Å | 8.0 x 300 mm | steel |
F6429202 | OHpak LB-806M | SEC | - | Polyhydroxy-methacrylate | ≥ 12,000 | 13 µm | 15,000 Å | 8.0 x 300 mm | steel |
F6709434 | OHpak LB-G 6B | SEC | - | Polyhydroxy-methacrylate | (guard) | 13 µm | - | 6.0 x 50 mm | steel |
Product details
Product name | Maximum pressure | Usual flow rate | Maximum flow rate | Temperature range | pH range | Salt concentration | Shipping solvent | USP | MW range | Exclusion limit | Info |
|---|---|---|---|---|---|---|---|---|---|---|---|
OHpak LB-802.5 | 5.5 MPa (55 bar) | 0.5 to 1.0 mL/min | 1.2 mL/min | 4 to 80°C | 3 to 10 | ≤ 0.5 M | H2O | L25, L38, L39, L89 | 500 - 10,000 | 10,000 | MALS noise ≤ 5.0 × 10-5 V |
OHpak LB-803 | 5.5 MPa (55 bar) | 0.5 to 1.0 mL/min | 1.2 mL/min | 4 to 80°C | 3 to 10 | ≤ 0.5 M | H2O | L37, L38, L39 | 1,000 - 100,000 | 100,000 | MALS noise ≤ 2.0 × 10-5 V |
OHpak LB-804 | 3.5 MPa (35 bar) | 0.5 to 1.0 mL/min | 1.2 mL/min | 4 to 80°C | 3 to 10 | ≤ 0.5 M | H2O | L38, L39 | 5,000 - 400,000 | 1,000,000 | MALS noise ≤ 5.0 × 10-5 V |
OHpak LB-805 | 3.5 MPa (35 bar) | 0.5 to 1.0 mL/min | 1.2 mL/min | 4 to 80°C | 3 to 10 | ≤ 0.5 M | H2O | L38, L39 | 100,000 - 1,000,000 | (4,000,000) | MALS noise ≤ 5.0 × 10-5 V |
OHpak LB-806 | 3.5 MPa (35 bar) | 0.5 to 1.0 mL/min | 1.2 mL/min | 4 to 80°C | 3 to 10 | ≤ 0.5 M | H2O | L38, L39 | 100,000 - (20,000,000) | (20,000,000) | MALS noise ≤ 5.0 × 10-5 V |
OHpak LB-806M | 3.5 MPa (35 bar) | 0.5 to 1.0 mL/min | 1.2 mL/min | 4 to 80°C | 3 to 10 | ≤ 0.5 M | H2O | L38, L39 | 500 - (20,000,000) | (20,000,000) | MALS noise ≤ 2.0 × 10-5 V |
OHpak LB-G 6B | - | - | - | 4 to 80°C | 3 to 10 | ≤ 0.5 M | H2O | L38, L39 | - | - |
Applications
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General OHpak LB-800
Calibration Curves for LB-800 Series (Aqueous Eluent: Pullulan)
Calibration Curves for LB-800 Series (DMF Eluent: PEG, PEO)
Analysis OHpak LB-800
Absolute Molecular Weight Determination of Heparin (LB-806M)
Absolute Molecular Weight Determination of PMMA (LB-802.5)
Absolute Molecular Weight Determination of Sodium Alginate (LB-806M)
Related products
Shodex STANDARD P-82 (Pullulan) is a calibration standard kit for aqueous size exclusion chromatography.
Pullulan is an unbranched polysaccharide: units of maltotriose are connected with α-1,6 bond.
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Easy to prepare
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For aqueous SEC (GFC)
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Unbranched pullulan standard
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High solubility in water eliminates the possibility of recrystallization
STANDARD P-82 kit consists of 8 vials with different molecular weight standards of 200 mg each:
MW 800.000, 400.000, 200.000, 100.000, 50.000, 20.000, 10.000, 5.000
