Pullulan calibration standard
water-soluble unbranched polysaccharide
The calibration kit for aqueous SEC
Product information
Shodex STANDARD P-82 (Pullulan) is a calibration standard for aqueous size exclusion chromatography.
Pullulan is a polysaccharide: units of maltotriose are connected with α-1,6 bond, and its chains are linear without branched chains.
The sample solution of this standard is easy to prepare and easy to handle, because it is stable without agglomeration, crystallization or electrical charge, and moreover pullulan is not adsorbed to column packing materials. This is useful as molecular weight standard for Size Exclusion Chromatography (SEC).
-
For aqueous SEC (GFC)
-
Unbranched pullulan standard
-
High solubility in water eliminates the possibility of recrystallization
In order to obtain a calibration curve, plot Mp and retention time using the same analysis condition as the target sample analysis. Relative molecular weight of unknown sample can be calculated by comparing the calibration curve with the measured results.
Pullulan kit: STANDARD P-82 (8 vials)
STANDARD P-82 kit consists of 8 kinds of different molecular weight standards of 200 mg each.
Note: Molecular weights (Mp, Mw/Mn) of a standard kit or each single type may vary depending on production lots. Always refer to the inspection certificate.
Product overview
Product code | Product name | Compound | Type | Content | Molecular weights (Mp) Range* | Mw/Mn* |
|---|---|---|---|---|---|---|
F8400000 | STANDARD P-82 | Pullulan | Kit | 0.2 g x 8 kinds | ~ 5,000 - 800,000 | - |
Product instruction
Sample Solution Preparation
(1) Recommended standard sample concentration is 0.1 - 0.5 % (w/v).
(2) Add solvent to standard and let it stand to swell. The time required for swelling and dissolution depends on molecular weight of a standard. Lower-molecular-weight standards swell and dissolve in a few hours. However, higher-molecular-weight standards require a longer time. Leave them in a refrigerator for half a day to a day until they fully swell.
(3) Once standard is fully swollen, gently stir the sample solution to let it completely dissolve and homogenize.
(4) Filter the prepared sample solutions using disposable 0.45 μm filters.
(5) Sample solutions should be stored in a refrigerator (about 4°C recommended) to prevent it from bacterial growth. Before storing the sample, adjust the sample pH between 5 and 7.
(6) Use refrigerated sample solutions within 1 week.
Drying
Standards contain a small amount of water. Dry standards before sample solution preparation to obtain desired concentrations with higher accuracy. Standards contain a small amount of water. If concentration with an exact accuracy is required, dry the standard before sample solution preparation. Below are example drying methods.
P-5 to P-100: Vacuum dry at 90°C for 6 hours or longer
P-200 to P-800: Leave in a desiccator containing phosphorus pentoxide
Detection
Pullulan has a very weak UV absorbance, thus a UV detectors is not suitable. Generally, a RI detector is used.
Storage
Store undissolved standards in a desiccator or an airtight container and refrigerate (about 4 °C recommended). Do not open reagent bottles immediately after taking out from the refrigerator. Wait opening until the bottle temperature reaches the room temperature.